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The extraction of water-soluble metabolites from serum samples was performed based on the Folch, Lees, and Sloane-Stanley procedure 33 and has been already described in previously published papers 34

Oxidative stress-induced glutathionylation has been recognized as a mechanism for induction and regulation of several proteins, including Grx (glutaredoxin), Trxs (thioredoxin) and GSTs, which are involved in environmental stress responses (Dixon et al

A more accurate assessment of these values was not possible due to low solubility of these compounds at high micromolar concentrations

Future studies on restoring NADH/NAD + redox balance could provide further insights into design of novel antidiabetic strategies

Nat Prod J 14